Protein ID Core - Home

The Protein ID core facility for the cancer center is situated in the Research III Building at the UC Davis Medical Center. The mission of the facility is to develop and establish a core of excellence for proteomics with emphasis on protein identification. Studying large protein complexes such as signalsomes, chromatins, transcriptional complexes, DNA repair complexes and DNA replication complexes are fundamental to our understanding of regulation of growth, survival, chemo-resistance and metastasis of cancer cells. Isolation of protein complexes with affinity copurification followed by protein identification using mass spectrometry has become a powerful strategy to discover protein-protein interaction. This facility, when fully established, will offer members opportunities to identify associated proteins either purified from the gel or derived directly from cellular lysates.

Our facility is equipped with the electrospray ionization mass spectrometer (LCQ-DECA, Thermo Electron Corporation) interfaced with a fully integrated multi-dimensional chromatography HPLC system (Paradigm MS4, Michrom Inc.) for microflow or nanoflow applications (high sensitivity) and on-line fragmentation, sequencing and identification of peptides, which enables the identification of protein with high sensitivity and accuracy, especially when dealing with proteins of high complexity.